The Cytiva Streptavidin Sepharose HP Affinity Resin, 5 mL (17511301) is a preswollen affinity chromatography medium designed for binding biotin and biotinylated substances. Purified streptavidin is immobilised on a highly cross-linked Sepharose High Performance agarose matrix, providing a versatile medium for creating affinity purification methods based on the strong streptavidin-biotin interaction.
Key Features
- High-affinity streptavidin ligand, with purified streptavidin from Streptomyces avidinii immobilised on Sepharose High Performance
- 5 mL preswollen resin format, supplied as chromatography medium in suspension for user-packed affinity purification workflows
- High biotin binding capacity, providing greater than 300 nmol biotin per mL of medium
- Strong binding of biotinylated proteins, with a capacity of 6 mg biotinylated bovine serum albumin per mL of medium
- Highly cross-linked spherical agarose matrix, providing good flow properties with high physical and chemical stability
- Fine 34 µm average particle size, characteristic of the Sepharose High Performance matrix
- Working pH stability from 2 to 10.5, supporting a broad range of affinity chromatography conditions
- Working temperature from 4°C to room temperature, covering common laboratory purification conditions
- Versatile biotin-based affinity chemistry, allowing biotinylated substances to serve as ligands for customised affinity purification
- Suitable for antigen purification workflows, including capture using biotinylated antibody-antigen complexes
- Suitable for packing into compatible laboratory columns, including the Cytiva Tricorn 5/50 column referenced by the manufacturer
- Supplied in 20% ethanol, with refrigerated storage specified between 4°C and 8°C
Benefits
- Enables flexible development of affinity purification methods by using virtually any suitable biotinylated substance as the immobilised binding ligand.
- Supports effective capture of biotinylated biomolecules through the exceptionally strong streptavidin-biotin interaction.
- Provides high binding capacity in a compact 5 mL resin quantity for laboratory-scale chromatography applications.
- Allows users to pack the resin into an appropriate column configuration for their individual purification workflow.
- Supports specialised antigen purification and iminobiotin-based methods in addition to conventional biotin affinity applications.
Why Choose the Cytiva Streptavidin Sepharose HP Affinity Resin, 5 mL (17511301)?
This affinity resin combines immobilised streptavidin with a stable Sepharose High Performance matrix to provide high-capacity capture of biotin and biotinylated substances in a flexible bulk-resin format. Buy Cytiva Streptavidin Sepharose HP from John Morris Group for biotin-based affinity chromatography and biomolecule purification workflows in Australia.
