The Cytiva PD SpinTrap G-25 Desalting Columns, Pack of 50 (28918004) are prepacked single-use microspin columns for rapid cleanup of proteins and other biomolecules. Each column contains Sephadex G-25 DNA Grade F and is designed for desalting, buffer exchange and removal of low-molecular-weight compounds from samples in the 100–180 µL range.
Key Features
- Rapid single-use sample cleanup, designed for desalting, buffer exchange and removal of low-molecular-weight compounds
- Sephadex G-25 DNA Grade F matrix, providing size-based group separation of high- and low-molecular-weight substances
- 85–260 µm particle size range, with approximately 600 µL of packed resin per column
- Recommended for 100–180 µL samples, with a maximum sample volume of 180 µL per column
- High desalting capacity above 85%, supporting efficient removal of salts and other small compounds
- Exclusion limit of Mr 5,000, separating larger biomolecules from lower-molecular-weight substances
- Typical sample recovery of 70–90%, depending on the protein or other biomolecule being processed
- Broad working pH range from 2 to 13, suitable for commonly used laboratory buffers
- Designed for standard microcentrifugation, with processing performed at 800 × g for convenient parallel sample preparation
- Polypropylene barrel with polyethylene frits, forming the disposable 1,000 µL column body
Note: These columns are intended for research use only and are not for clinical or in vitro diagnostic procedures.
Benefits
- Speeds up small-volume sample preparation by combining desalting and buffer exchange in a simple centrifugation workflow.
- Allows multiple protein or biomolecule samples to be processed in parallel using standard laboratory microcentrifuge equipment.
- Minimises sample dilution during cleanup, supporting downstream analytical workflows where sample concentration is important.
- Provides predictable separation performance through a defined exclusion limit, desalting capacity and sample-volume range.
- Reduces column preparation work through a prepacked, disposable format supplied with matching collection tubes.
Why Choose the Cytiva PD SpinTrap G-25 Desalting Columns, Pack of 50 (28918004)?
The Cytiva PD SpinTrap G-25 provides a convenient prepacked format for rapid small-scale desalting, buffer exchange and biomolecule cleanup using a standard microcentrifuge. Buy from John Morris Group for Cytiva sample preparation and chromatography products in Australia.
