The Cytiva Ni Sepharose 6 Fast Flow Affinity Resin, 25 mL (17531801) is a precharged immobilised metal affinity chromatography medium for purification of histidine-tagged proteins. It uses a highly cross-linked 6% spherical agarose matrix with an average particle size of 90 µm and a dynamic binding capacity of approximately 40 mg histidine-tagged protein per mL of medium.
Key Features
- Precharged nickel affinity medium, designed for immobilised metal affinity chromatography of histidine-tagged proteins
- Highly cross-linked 6% spherical agarose matrix, providing the base support for the chromatography medium
- Average particle size of 90 µm, characteristic of the Fast Flow resin format
- Dynamic binding capacity of approximately 40 mg/mL, measured with histidine-tagged protein under Cytiva's specified test conditions
- Metal ion capacity of approximately 15 µmol Ni²?/mL, providing the immobilised nickel sites used for affinity binding
- Recommended linear flow velocity of 150 cm/h, supporting routine purification workflows
- Maximum linear flow velocity up to 600 cm/h, specified under Cytiva's defined XK 16/20 test conditions
- Working pH range from 3 to 12, with regeneration stability from pH 2 to 14
- Supplied preswollen in a 25 mL pack, ready for packing into a suitable chromatography column
Benefits
- Purify histidine-tagged recombinant proteins using a nickel-charged affinity medium designed for IMAC workflows.
- Scale column packing to suit laboratory requirements using the loose-resin format.
- Support high protein loading with an approximate dynamic binding capacity of 40 mg/mL under specified conditions.
- Optimise purification throughput across a broad range of compatible linear flow velocities.
- Work across varied buffer conditions with a working pH stability range from 3 to 12.
Why Choose the Cytiva Ni Sepharose 6 Fast Flow Affinity Resin, 25 mL (17531801)?
The Cytiva Ni Sepharose 6 Fast Flow resin combines a nickel-charged agarose matrix, high protein-binding capacity and flexible column-packing format for purification of histidine-tagged proteins. Buy from John Morris Group for genuine Cytiva affinity chromatography resins and protein purification consumables.
