The Cytiva HiTrap Q HP Anion Exchange Columns, 5 × 1 mL (17115301) are prepacked strong anion exchange chromatography columns containing Q Sepharose High Performance resin for high-resolution protein purification. Each 1 mL column uses a cross-linked agarose matrix with approximately 34 µm particles and provides a dynamic binding capacity of approximately 70 mg BSA per mL of resin.
Key Features
- Strong anion exchange chemistry, using a quaternary ammonium Q charged group for binding negatively charged biomolecules
- Q Sepharose High Performance resin, based on a spherical cross-linked agarose matrix for high-resolution ion exchange chromatography
- Fine approximately 34 µm particles, supporting efficient chromatographic separations in the HiTrap format
- Approximately 70 mg BSA/mL resin dynamic binding capacity, measured under Cytiva's specified test conditions
- 1 mL prepacked column volume, with five ready-to-use columns supplied in each pack
- Recommended operating flow rate of 1 mL/min, with a maximum operating flow rate of 4 mL/min for the 1 mL format
- Broad operational pH range from 2 to 12, with CIP stability from pH 2 to 14
- Compact 7 × 25 mm column format, providing a convenient prepacked configuration for laboratory-scale purification and method development
- Chemically stable chromatography medium, compatible with commonly used aqueous buffers and specified cleaning and denaturing solutions
Benefits
- Reduces preparation time by providing prepacked columns that are ready for ion exchange purification workflows.
- Supports high-resolution purification through the small-particle Q Sepharose High Performance matrix.
- Provides useful protein-binding capacity in a compact 1 mL format for screening, method development and laboratory purification.
- Allows flexible method conditions through a broad operational pH range and compatibility with commonly used chromatography buffers.
- Simplifies scale-up within the HiTrap Q HP family by using the same ion exchange chemistry in defined prepacked formats.
Why Choose the Cytiva HiTrap Q HP Anion Exchange Columns, 5 × 1 mL (17115301)?
The Cytiva HiTrap Q HP combines strong anion exchange chemistry with fine Q Sepharose High Performance resin in a convenient prepacked 1 mL format for high-resolution protein purification and method development. Buy from John Morris Group for Cytiva ion exchange chromatography columns and protein purification solutions in Australia.
