The Cytiva HiTrap Q FF Anion Exchange Columns, 5 × 1 mL (17505301) are prepacked ion exchange chromatography columns for protein purification by charge. Each 1 mL column contains Q Sepharose Fast Flow strong anion exchange resin with a 90 µm median particle size.
Key Features
- Strong anion exchange chemistry, using Q Sepharose Fast Flow resin for separation of biomolecules according to charge
- Five prepacked 1 mL columns per pack, providing ready-to-use columns for repeated ion exchange workflows
- Dynamic binding capacity of 120 mg HSA per mL of resin, providing a defined reference capacity for the Q Sepharose Fast Flow medium
- Loading range of 1–120 mg HSA, as specified by Cytiva for the 1 mL HiTrap Q FF format
- Recommended flow rate up to 1 mL/min, supporting routine operation of the 1 mL column format
- Maximum pressure over the packed bed of 0.3 MPa, providing a defined operating pressure limit
- Broad operational pH range from 2 to 12, supporting purification across varied buffer conditions
- 90 µm median particle size, characteristic of the Sepharose Fast Flow resin platform
- Compact 7 × 25 mm packed-bed dimensions, providing a 1 mL bed volume in the HiTrap format
Benefits
- Perform anion exchange chromatography without manually packing the separation medium.
- Purify proteins and other charged biomolecules using a strong anion exchanger in a compact prepacked format.
- Develop purification methods across a broad operational pH range from 2 to 12.
- Support routine laboratory workflows with a recommended flow rate up to 1 mL/min.
- Process repeated separations conveniently with five prepacked columns supplied in each pack.
Why Choose the Cytiva HiTrap Q FF Anion Exchange Columns, 5 × 1 mL (17505301)?
The Cytiva HiTrap Q FF combines Q Sepharose Fast Flow strong anion exchange resin with a convenient prepacked 1 mL format for protein purification by charge. Buy from John Morris Group for genuine Cytiva chromatography columns and consumables for laboratory purification workflows.
