The Cytiva HiTrap NHS-Activated HP Column, 1 × 5 mL (17071701), is a preactivated affinity chromatography column for covalent coupling of ligands containing primary amines. The 5 mL column is packed with NHS-activated Sepharose High Performance medium and supports convenient preparation of customised affinity columns.
Key Features
- Single prepacked 5 mL column, supplied as a ready-to-use 1 × 5 mL configuration for customised affinity chromatography
- Designed for primary amine coupling, enabling immobilisation of proteins and other suitable ligands containing primary amino groups
- NHS-activated Sepharose High Performance medium, providing preactivated chemistry for covalent ligand attachment
- Approximately 10 µmol NHS groups per mL of medium, providing the activated groups used for ligand coupling
- 10-atom spacer arm, separating the immobilised ligand from the agarose matrix
- Fine 34 µm mean particle size, based on highly cross-linked spherical agarose
- Recommended flow rate of 5 mL/min, with a maximum flow rate of 20 mL/min for the 5 mL column
- Coupling conditions from pH 6.5 to 9, with coupling possible at room temperature or 4 °C
- Broad pH stability from 3 to 12, depending on the properties of the coupled ligand
- Compact 5 mL column bed, measuring 1.6 cm internal diameter × 2.5 cm bed height
- Column hardware rated to 5 bar, equivalent to 0.5 MPa
Benefits
- Simplifies preparation of customised affinity columns by providing the activated chromatography medium in a prepacked format.
- Reduces ligand immobilisation steps by allowing direct coupling through primary amino groups.
- Supports flexible affinity method development using a user-selected ligand appropriate to the target biomolecule.
- Provides practical flow characteristics for rapid coupling, washing, and subsequent chromatography workflows.
- Allows coupling under near-neutral conditions, helping accommodate a broad range of suitable protein ligands.
Why Choose the Cytiva HiTrap NHS-Activated HP Column, 1 × 5 mL (17071701)?
The Cytiva HiTrap NHS-Activated HP 5 mL column combines preactivated NHS chemistry with a convenient prepacked High Performance agarose format for preparing customised affinity columns. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
