The Cytiva HiTrap Desalting Columns, 5 × 5 mL (17140801) are prepacked size exclusion chromatography columns for desalting and buffer exchange. Each 5 mL column is packed with Sephadex G-25 Superfine and accommodates sample volumes from 0.25 to 1.5 mL.
Key Features
- Five prepacked 5 mL columns per pack, providing a convenient format for repeated desalting and buffer exchange workflows
- Designed for 0.25 to 1.5 mL sample volumes, supporting small-scale sample preparation
- Recommended flow rate of 5 mL/min, with a maximum specified flow rate of 15 mL/min
- Prepacked with Sephadex G-25 Superfine, a size exclusion chromatography resin used for desalting and buffer exchange
- Exclusion limit greater than 5 kDa, as specified by Cytiva for the packed resin
- Broad regular-use pH range of 2 to 13, supporting operation across varied buffer conditions
- 40 µm median particle size, based on the cumulative volume distribution
- Ready-to-use HiTrap 5 mL column format, eliminating the need to manually pack the chromatography resin
Benefits
- Streamline desalting and buffer exchange by using prepacked columns rather than preparing chromatography beds manually.
- Process small sample volumes from 0.25 to 1.5 mL in a standardised 5 mL column format.
- Support efficient laboratory workflows with a recommended flow rate of 5 mL/min and maximum flow rate of 15 mL/min.
- Work across a broad range of buffer conditions with a regular-use pH range from 2 to 13.
- Keep multiple columns available for repeated experiments with five 5 mL columns supplied in each pack.
Why Choose the Cytiva HiTrap Desalting Columns, 5 × 5 mL (17140801)?
The Cytiva HiTrap Desalting Columns provide a ready-to-use Sephadex G-25 Superfine format for small-scale desalting and buffer exchange, with sample volumes from 0.25 to 1.5 mL. Buy from John Morris Group for genuine Cytiva chromatography consumables for your laboratory purification workflows.
