The Cytiva HiTrap Protein A HP Column, 1 × 5 mL (17040301) is a prepacked affinity chromatography column containing Protein A Sepharose High Performance resin for purification of antibodies such as human IgG. The 5 mL column provides a typical human IgG loading range of 5 to 125 mg per column.
Key Features
- Prepacked for antibody affinity purification, using Protein A Sepharose High Performance chromatography resin
- Single 5 mL column format, supplied ready for routine laboratory antibody purification workflows
- Typical human IgG loading range of 5 to 125 mg, supporting preparative antibody purification in the 5 mL format
- Approximately 125 mg binding capacity per column, based on the specified capacity of Protein A Sepharose High Performance
- Small 34 µm spherical particles, helping minimise dilution of the eluted antibody peak
- Recommended flow rate of 2.5 mL/min, with a maximum flow rate of 20 mL/min for the 5 mL column
- Regular-use pH range from 3 to 10, supporting commonly used antibody purification conditions
- Biocompatible polypropylene construction, designed not to interact with biomolecules
- Compact 1.6 × 2.5 cm column dimensions, corresponding to the 5 mL HiTrap column format
- 5 bar hardware pressure limit, equivalent to 0.5 MPa
- Protein A affinity selectivity, enabling capture of antibodies through interaction with the Fc region of compatible IgG molecules
- Ready-to-connect HiTrap format, supporting convenient use in compatible laboratory chromatography workflows
Benefits
- Reduces column preparation time by providing the Protein A affinity medium in a prepacked, ready-to-use format.
- Supports efficient purification of human IgG and other compatible antibodies using established Protein A affinity chromatography.
- Helps produce concentrated elution peaks through the small particle size of the High Performance resin.
- Provides useful preparative capacity for laboratory antibody purification with a 5 mL bed volume.
- Supports reproducible chromatography workflows with a standardised prepacked column format.
Why Choose the Cytiva HiTrap Protein A HP Column, 1 × 5 mL (17040301)?
The Cytiva HiTrap Protein A HP Column combines Protein A Sepharose High Performance resin with a convenient 5 mL prepacked format for routine antibody purification. Buy Cytiva HiTrap Protein A HP columns from John Morris Group for affinity chromatography and IgG purification workflows in Australia.
