The Cytiva HisTrap HP IMAC Column, 5 mL (17524801) is a prepacked affinity chromatography column containing Ni Sepharose High Performance resin for purification of histidine-tagged proteins by immobilised metal ion affinity chromatography. The single 5 mL column provides a typical loading range of 5 to 200 mg of His-tagged protein per column.
Key Features
- Prepacked for His-tagged protein purification, using Ni Sepharose High Performance affinity resin
- Single 5 mL column format, supplied as one ready-to-use HisTrap HP column
- High protein loading capability, with a typical loading range of 5 to 200 mg of His-tagged protein per column
- High binding capacity resin, providing at least 40 mg of His-tagged protein per mL of medium
- Small 34 µm particles, supporting high-resolution affinity purification
- Recommended flow rate of 5 mL/min, with a maximum flow rate of 20 mL/min for the 5 mL format
- Broad regular-use pH range from 3 to 12, supporting varied purification conditions
- Biocompatible polypropylene column construction, designed to avoid interaction with biomolecules
- Compact 1.6 × 2.5 cm column dimensions, corresponding to the 5 mL HiTrap column format
- 5 bar hardware pressure limit, equivalent to 0.5 MPa
- Nickel-charged affinity chemistry, selectively retaining proteins with exposed histidine groups
- Flexible chromatography operation, suitable for use with syringes, pumps and compatible chromatography systems
Benefits
- Supports high-yield purification by combining a 5 mL bed volume with high-capacity Ni Sepharose High Performance resin.
- Helps achieve high-resolution separations through the small particle size of the packed affinity medium.
- Reduces column preparation work because the affinity medium is supplied prepacked and ready for purification workflows.
- Allows purification conditions to be adapted across a broad working pH range and a range of commonly used chromatography reagents.
- Provides flexibility for manual or system-based purification workflows using syringes, pumps or compatible chromatography systems.
Why Choose the Cytiva HisTrap HP IMAC Column, 5 mL (17524801)?
The Cytiva HisTrap HP IMAC Column combines high-capacity Ni Sepharose High Performance resin with a convenient prepacked 5 mL format for efficient purification of His-tagged proteins. Buy Cytiva HisTrap HP columns from John Morris Group for protein purification and affinity chromatography workflows in Australia.
