The Cytiva HisTrap FF IMAC Columns, 5 × 5 mL (17525501) are ready-to-use affinity chromatography columns prepacked with Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilised metal ion affinity chromatography. The pack contains five 5 mL columns with a typical loading range of 5 to 200 mg of His-tagged protein per column.
Key Features
- Prepacked for His-tagged protein purification, using precharged Ni Sepharose 6 Fast Flow affinity medium
- Five 5 mL columns per pack, providing a convenient format for repeated preparative purification workflows
- Typical loading range of 5 to 200 mg per column, supporting preparative purification of His-tagged proteins
- Approximately 40 mg/mL dynamic binding capacity, providing high protein-binding capability with Ni Sepharose 6 Fast Flow
- Highly cross-linked 6% spherical agarose matrix, providing the foundation for the Ni Sepharose 6 Fast Flow medium
- 90 µm average particle size, supporting high flow rates for efficient purification
- Recommended flow rate of 5 mL/min, with a maximum flow rate of 20 mL/min for the 5 mL column format
- Broad working pH range from 3 to 12, allowing use across varied purification conditions
- Biocompatible polypropylene column construction, designed for biomolecule purification workflows
- Compact 1.6 × 2.5 cm column dimensions, corresponding to the 5 mL HisTrap format
- 5 bar column hardware pressure limit, equivalent to 0.5 MPa
- Compatible with commonly used purification additives, including buffers, reducing agents, denaturants and detergents
Benefits
- Reduces preparation time because the affinity medium is supplied prepacked and ready for chromatography.
- Supports efficient preparative purification through the combination of high binding capacity and high permissible flow rates.
- Provides flexibility for different His-tagged protein purification conditions across a broad working pH range.
- Helps maintain reliable capture performance through the precharged nickel affinity chemistry of Ni Sepharose 6 Fast Flow.
- Allows convenient operation with a syringe, laboratory pump or compatible chromatography system such as an ÄKTA system.
Why Choose the Cytiva HisTrap FF IMAC Columns, 5 × 5 mL (17525501)?
These prepacked columns combine Ni Sepharose 6 Fast Flow with a 5 mL bed volume and high flow capability for efficient preparative purification of His-tagged proteins. Buy Cytiva HisTrap FF columns from John Morris Group for protein purification and affinity chromatography workflows in Australia.
