The Cytiva HisTrap FF Crude IMAC Columns, 5 × 5 mL (17528601), are ready-to-use prepacked columns for purification of His-tagged recombinant proteins by immobilised metal affinity chromatography. Packed with precharged Ni Sepharose 6 Fast Flow medium, they allow unclarified cell lysate to be loaded directly after thorough cell disruption.
Key Features
- Five prepacked 5 mL columns, supplied as a 5 × 5 mL configuration for repeat protein purification workflows
- Direct processing of unclarified lysate, allowing thoroughly disrupted samples to be loaded without prior centrifugation and filtration
- Precharged Ni Sepharose 6 Fast Flow medium, designed for immobilised metal affinity purification of His-tagged proteins
- High dynamic binding capacity, approximately 40 mg of His-tagged protein per mL of chromatography medium
- Fast 5 mL/min recommended flow rate, with a maximum flow rate of 20 mL/min for the 5 mL column format
- 90 µm average bead size, providing the flow characteristics of Ni Sepharose 6 Fast Flow medium
- Compact 5 mL column bed, measuring 1.6 cm internal diameter × 2.5 cm bed height
- Column hardware rated to 5 bar, equivalent to 0.5 MPa
Benefits
- Shortens sample preparation by enabling purification directly from thoroughly disrupted, unclarified cell lysates.
- Reduces the need for centrifugation and filtration steps before chromatographic purification.
- Supports efficient capture of His-tagged proteins with approximately 40 mg/mL dynamic binding capacity.
- Enables rapid purification workflows through the high-flow properties of the Fast Flow chromatography medium.
- Provides five ready-to-use columns for repeat experiments, method development, or multiple purification runs.
Why Choose the Cytiva HisTrap FF Crude IMAC Columns, 5 × 5 mL (17528601)?
The Cytiva HisTrap FF Crude 5 × 5 mL columns combine high-capacity Ni Sepharose 6 Fast Flow medium with the ability to process unclarified lysates directly, helping simplify His-tagged protein purification. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
