The Cytiva His Buffer Kit for Histidine-Tagged Protein Purification (11003400) provides premade buffer concentrates for purification of histidine-tagged proteins. The kit contains two 100 mL bottles of 8× phosphate buffer concentrate at pH 7.4 and one 100 mL bottle of 2 M imidazole at pH 7.4.
Key Features
- Premade buffer concentrates for His-tag purification, reducing the need to prepare core phosphate and imidazole stock solutions from individual reagents
- Two 100 mL bottles of 8× phosphate buffer concentrate, supplied at pH 7.4 for preparation of purification buffers
- One 100 mL bottle of 2 M imidazole solution, supplied at pH 7.4 for preparing binding and elution conditions
- Supports adjustable imidazole concentrations, allowing binding and elution buffers to be prepared with different imidazole levels
- Designed for histidine-tagged protein purification, supporting affinity chromatography workflows using nickel-based His-tag media
- Compatible with His GraviTrap columns, providing premade concentrates for the recommended purification workflow
- Compatible with HisTrap and His SpinTrap columns, extending use across multiple Cytiva His-tag purification formats
Benefits
- Reduce routine buffer preparation by starting with premade concentrated solutions.
- Adjust imidazole concentration to optimise binding, washing and elution conditions for different histidine-tagged proteins.
- Maintain consistent phosphate and imidazole stock concentrations across repeated purification workflows.
- Use one buffer system across several Cytiva His-tag chromatography column formats.
Why Choose the Cytiva His Buffer Kit for Histidine-Tagged Protein Purification (11003400)?
The Cytiva His Buffer Kit provides concentrated phosphate buffer and high-concentration imidazole stocks for preparing buffers used in histidine-tagged protein purification workflows. Buy from John Morris Group for genuine Cytiva protein purification consumables and chromatography accessories.
