The Cytiva Glutathione Sepharose 4B GST Resin, 10 mL (17075601) is an affinity chromatography medium for purification of glutathione S-transferase (GST) and GST-tagged proteins. It uses glutathione immobilised through a 10-carbon linker on a 4% agarose matrix and is supplied as a 10 mL pack for laboratory protein purification workflows.
Key Features
- Designed for GST affinity purification, using immobilised glutathione for selective capture of GST and GST-tagged fusion proteins
- 4% agarose matrix, providing the base chromatography medium for affinity purification
- Glutathione ligand with a 10-carbon linker arm, providing the affinity interaction used for GST capture
- Ligand concentration of 200–400 µmol/g, measured per gram of washed and dried medium
- Binding capacity above 5 mg GST/mL medium, with actual binding dependent on protein and loading conditions
- Average particle size of 90 µm, supporting laboratory-scale affinity chromatography workflows
- Recommended flow rate below 75 cm/h, specified using water at room temperature
- Stable across pH 4 to 13, providing a broad working range for purification and cleaning procedures
- Compatible with commonly used aqueous buffers, supporting routine GST purification protocols
- Supplied as a 10 mL resin pack, suitable for preparation of affinity chromatography beds as required
Benefits
- Enables selective capture of GST-tagged proteins through established glutathione affinity chemistry.
- Allows users to prepare column beds or batch purification formats according to their required sample scale.
- Supports efficient laboratory workflows with defined binding, particle-size and flow characteristics.
- Offers broad pH and chemical stability for flexibility across purification and cleaning procedures.
- Provides a bulk resin format that can be adapted to different GST-tagged protein purification requirements.
Why Choose the Cytiva Glutathione Sepharose 4B GST Resin, 10 mL (17075601)?
The Cytiva Glutathione Sepharose 4B provides established glutathione affinity chemistry in a flexible bulk resin format for purification of GST-tagged proteins. Buy from John Morris Group for Cytiva affinity chromatography resins and protein purification solutions in Australia.
