The ÄKTA pure 25 M Chromatography System (29018226) is a flexible liquid chromatography system for preparative protein, peptide and nucleic acid purification in research applications. This 25 M configuration combines a system pump flow rate of 0.001 to 25 mL/min, a pressure range of 0 to 20 MPa and UV monitor U9-M for multiwavelength detection from 190 to 700 nm.
Key Features
- Preparative ÄKTA pure 25 platform, designed for flexible research-scale purification using flow rates up to 25 mL/min
- Multiwavelength UV detection, with UV monitor U9-M covering 190 to 700 nm in 1 nm steps
- Up to three simultaneous UV wavelengths, supporting richer monitoring of proteins, peptides, nucleic acids and other biomolecules
- High-pressure system pump, with 0.001 to 25 mL/min operation and up to 50 mL/min during column packing
- 20 MPa operating pressure range, equivalent to 200 bar or 2900 psi for compatible chromatography applications
- UNICORN software control, using UNICORN 7.6 or later for method control, run monitoring and result evaluation
- Included 1.4 mL mixer, with optional 0.6 mL and 5 mL mixer chambers available for workflow flexibility
- Gradient formation capability, with 0.1 to 25 mL/min gradient flow range and ± 0.6% gradient composition accuracy under stated conditions
- Rotary valve architecture, with a standard injection valve and optional inlet, mixer by-pass, loop, column, pH, outlet and versatile valve functions
- Conductivity and temperature monitoring, with 0.01 to 999.99 mS/cm conductivity reading range and 0°C to 99°C temperature reading range
- Modular system design, allowing hardware options such as sample pump, pH monitoring, fraction collection and additional valves to be configured for workflow needs
Benefits
- Supports research-scale purification while maintaining a compact system footprint for bench or cold cabinet placement.
- Provides broader UV monitoring flexibility than fixed-wavelength ÄKTA pure L configurations.
- Helps laboratories analyse samples at multiple wavelengths during the same run for more informative purification data.
- Supports reproducible chromatography workflows with controlled flow, pressure, UV, conductivity and temperature data.
- Can be expanded with optional modules as sample application, fraction collection or automation needs grow.
Why Choose the ÄKTA pure 25 M Chromatography System (29018226)?
The ÄKTA pure 25 M Chromatography System (29018226) is a strong choice for research laboratories needing flexible purification capability with multiwavelength UV detection and a 20 MPa system pressure range. John Morris Group supplies Cytiva chromatography systems across Australia and can help configure the system, accessories and software around your purification workflow.
