The VH3 Ligand ELISA Kit, 96-Well, 1 Unit (29737000) is an enzyme-linked immunosorbent assay kit for measuring residual ligand leakage when using MabSelect VH3 protein A chromatography resin. Supplied as a 1 unit kit with a 96-well strip-plate and required assay reagents, it is designed to support VH3 ligand impurity detection in downstream bioprocess samples.
Key Features
- Matched VH3 protein A ligand included, reducing the need to source a separate standard or develop a custom protocol
- Designed for MabSelect VH3 resin workflows, supporting residual ligand leakage testing with MabSelect VH3 protein A chromatography resin
- 96-well strip-plate kit format, supplied as one unit with the required reagents for the assay
- High sensitivity measurement, with LLD and LLQ values of 10 pg/mL in 2 mg/mL IgG
- Broad assay range, measuring from 18 pg/mL to 4.5 ng/mL to help reduce sample dilution guesswork
- High IgG tolerance, with VH3 ligand quantified within specification in samples containing up to 30 mg/mL IgG
- Controlled assay reproducibility, with intra-assay and inter-assay CV specifications below 20%
- Standard recovery range of 80% to 120%, supporting reliable calibration and assay performance checks
Benefits
- Helps laboratories monitor residual protein A ligand during development and manufacture of antibody-based biopharmaceuticals.
- Reduces assay setup complexity by supplying a resin-matched VH3 ligand standard and the required kit reagents.
- Supports testing of late-process samples where residual ligand levels may be very low.
- Provides reproducible impurity quantification across assay runs and plates.
- Helps minimise sourcing steps by combining the strip-plate, buffers, detection antibody, substrate, stop solution, sealer and standard in one kit.
Why Choose the VH3 Ligand ELISA Kit, 96-Well, 1 Unit (29737000)?
This VH3 ligand ELISA kit is a purpose-built assay kit for residual VH3 ligand testing in MabSelect VH3 resin workflows. John Morris Group supplies Cytiva bioprocessing products across Australia, helping laboratories source verified assay consumables for impurity detection and process development.
