The Dissociation Enzyme Mix A, Lung, 25 Reactions (29740243) is an enzymatic kit for lung tissue dissociation in single-cell sample preparation workflows. Designed for use at 4°C or 37°C, it supports both manual tissue dissociation and semi-automated disaggregation using the VIA Extractor tissue disaggregator.
Key Features
- Optimised for lung tissue dissociation, supporting preparation of viable single-cell suspensions from mouse lung samples
- Flexible temperature use, designed for dissociation at 4°C or 37°C depending on the chosen workflow
- Manual and semi-automated workflow compatibility, supporting bench-based digestion or VIA Extractor tissue disaggregator methods
- 25-reaction manual pack format, with each pack also supporting 50 reactions when used with the VIA Extractor workflow
- Defined manual sample guidance, using 110 to 150 mg mouse lung tissue cut into 5 mm pieces
- Defined VIA Extractor method settings, using 200 rpm for 60 min with a 2°C program temperature setting for cold dissociation
- Prepared with enzyme 3, enzyme 5 and enzyme 6, using workflow-specific volumes for manual or semi-automated digestion
- Specified straining workflow, using 70 µm filtration for VIA Extractor processing and 70 µm plus 40 µm filtration for manual lung processing
Benefits
- Helps laboratories prepare lung tissue for downstream single-cell analysis workflows.
- Reduces method development effort by providing a tissue-specific enzyme mix with documented protocol settings.
- Supports workflow flexibility where laboratories need either manual processing or VIA Extractor semi-automated disaggregation.
- Helps improve consistency by using defined sample size, temperature, time and reagent volume guidance.
- Supports cold processing conditions where reduced handling stress is important for sensitive samples.
Why Choose the Dissociation Enzyme Mix A, Lung, 25 Reactions (29740243)?
This dissociation enzyme mix A kit is a lung-specific reagent pack for controlled tissue disaggregation in single-cell sample preparation. John Morris Group supplies Cytiva consumables across Australia, helping laboratories source verified reagents for tissue disaggregation and molecular workflow preparation.
