The Amersham CyDye DIGE Cy2 Minimal Dye, 25 nmol (RPK0272) is a fluorescent labelling reagent for 2-D Fluorescence Difference Gel Electrophoresis protein analysis. This Cy2 minimal dye is supplied as 5 × 5 nmol and is designed for use in DIGE workflows where protein abundance differences are compared on the same 2-D electrophoresis gel.
Key Features
- Cy2 minimal dye format, supplied as 25 nmol total dye in 5 × 5 nmol units
- Designed for 2-D DIGE protein analysis, supporting detection of protein abundance differences in fluorescence difference gel electrophoresis
- Size- and charge-matched dye design, enabling co-migration of labelled protein samples within the gel
- Supports multicolour DIGE analysis, allowing up to three prelabelled protein samples and standards on the same 2-D electrophoresis gel
- Minimal labelling approach, intended for general 2-D applications where sufficient sample is available
- 50 µg protein per labelling reaction, based on the verified product specification for the Cy2 DIGE fluor
- Cold, dark storage requirement, with storage specified at -15 °C to -30 °C and protection from light
Benefits
- Helps reduce gel-to-gel variation by allowing differently labelled protein samples to be run together under the same electrophoresis conditions.
- Supports accurate within-gel matching by using dyes designed for co-migration of labelled samples.
- Provides a ready pack size for larger DIGE experimental designs requiring multiple Cy2 labelling reactions.
- Helps simplify comparative proteomics workflows using Amersham CyDye DIGE fluor minimal dye chemistry.
Why Choose the Amersham CyDye DIGE Cy2 Minimal Dye, 25 nmol (RPK0272)?
The Amersham RPK0272 CyDye DIGE Cy2 minimal dye is designed for protein labelling prior to 2-D DIGE separation, supporting comparative protein abundance analysis in research workflows. John Morris Group can help Australian laboratories source Cytiva and Amersham DIGE reagents for electrophoresis and protein analysis applications.
