The Amersham CyDye DIGE Cy3 Minimal Dye, 5 nmol (25801083) is a fluorescent labelling reagent for proteins prior to 2-dimensional separation in 2-D fluorescence difference gel electrophoresis. It is supplied as a single vial containing 5 nmol of Cy3 minimal dye for use in DIGE protein abundance comparison workflows.
Key Features
- Cy3 minimal dye format, designed for labelling proteins before 2-D fluorescence difference gel electrophoresis
- 5 nmol single vial supply, matching the verified component listing for part 25801083
- Size- and charge-matched DIGE dye design, supporting comparison of protein abundance differences in 2-D DIGE workflows
- DMF reconstitution procedure, with 5 µL anhydrous DMF producing a 1 mM Cy3 dye solution
- Absorption maximum of 553 ± 3 nm, matching the verified Cy3 NHS ester characteristic in DMF
- Emission maximum of 572 ± 5 nm, supporting Cy3 fluorescence detection after protein labelling
- Storage at -15°C to -30°C, with protection from light and dark storage required
- Supplied with protocol documentation, including the product specification sheet and protocol booklet for 2-D DIGE
Note: This product is intended for research use only and is not for clinical or in vitro diagnostic procedures.
Benefits
- Supports detection of protein abundance differences in 2-D DIGE experiments.
- Helps enable comparison of CyDye-labelled protein samples within fluorescence gel electrophoresis workflows.
- Provides a dedicated Cy3 minimal dye option for laboratories using DIGE labelling protocols.
- Supports controlled protein labelling through the specified DMF reconstitution procedure.
- Helps laboratories maintain matched dye selection across Cy2, Cy3 and Cy5 DIGE workflows.
Why Choose the Amersham CyDye DIGE Cy3 Minimal Dye, 5 nmol (25801083)?
The Amersham CyDye DIGE Cy3 Minimal Dye, 5 nmol (25801083) provides a verified single-vial Cy3 minimal dye format for protein labelling before 2-D DIGE analysis. John Morris Group can support Australian laboratories with Amersham CyDye DIGE fluors and related electrophoresis reagents.
