The 2-D Quant Kit, 500 Assays (80648356) is a protein quantitation kit for samples prepared for electrophoresis techniques. It is designed for accurate determination of protein concentration in samples prepared for 2-D electrophoresis, SDS-PAGE or IEF, including samples containing common interfering preparation reagents.
Key Features
- Protein quantitation for electrophoresis samples, suitable for 2-D electrophoresis, SDS-PAGE and IEF workflows
- 500 assay kit format, supplied for laboratories processing multiple protein samples
- 0 to 50 µg linear protein range, with a recommended sample volume range of 1 to 50 µL
- Colourimetric copper-based assay, measuring unbound copper ion after proteins are precipitated and resuspended
- Compatible with common interfering reagents, including SDS, DTT, urea, thiourea, CHAPS, Pharmalyte and IPG Buffer at tested concentrations
- 480 nm absorbance readout, allowing measurement with a visible light spectrophotometer
- Supplied with BSA standard solution, supporting preparation of a protein standard curve
- 4 to 8°C storage, matching the manufacturer storage guidance for the kit
Note: For research use only. Not recommended or intended for diagnosis of disease in humans or animals.
Benefits
- Helps quantify protein directly in electrophoresis sample solutions that may interfere with many common protein assays.
- Supports consistent sample loading for comparative electrophoresis workflows.
- Reduces the need to change sample preparation conditions before protein concentration measurement.
- Provides a complete reagent kit format for routine protein analysis and sample preparation laboratories.
Why Choose the 2-D Quant Kit, 500 Assays (80648356)?
The 2-D Quant Kit, 500 Assays (80648356) is suited to laboratories preparing protein samples for electrophoresis where detergents, reductants, chaotropes or carrier ampholytes may affect standard assays. John Morris Group supplies protein sample preparation products in Australia for laboratories requiring reliable consumables for electrophoresis and protein analysis workflows.
