The GST SpinTrap Columns, 50 x 50 µL (28952359) are prepacked microspin affinity columns for rapid small-scale purification of GST-tagged proteins. Each column contains 50 µL of Glutathione Sepharose 4B and is designed for direct purification from clarified or unclarified cell lysates using a standard microcentrifuge.
Key Features
- Prepacked Glutathione Sepharose 4B medium, supplied in a convenient microspin format for GST-tagged protein purification
- 50 x 50 µL column pack, providing 50 prepacked columns with 50 µL bed volume per column
- Up to 500 µg rGST capacity per column, supporting rapid small-scale purification and expression screening
- Glutathione ligand chemistry, with a 10-carbon linker arm and 7 to 15 µmol glutathione/mL medium ligand concentration
- 4% agarose matrix, with an average bead size of 90 µm for Glutathione Sepharose 4B affinity capture
- 900 µL column volume, using a polypropylene barrel with polyethylene frits
- Mild elution conditions, using reduced glutathione to help preserve GST-tagged protein functionality
- Broad pH stability, specified from pH 4 to 13 for the medium
Benefits
- Supports rapid purification of GST-tagged proteins from clarified or unclarified cell lysates.
- Enables multiple small-scale samples to be purified in parallel during expression screening.
- Helps users evaluate media, growth temperature, culture density, induction conditions and other expression variables.
- Provides a ready-to-use spin column workflow without packing chromatography columns manually.
- Can deliver high-purity GST-tagged protein in a single affinity purification step under suitable conditions.
Why Choose the GST SpinTrap Columns, 50 x 50 µL (28952359)?
The GST SpinTrap Columns, 50 x 50 µL (28952359) provide a compact, ready-to-use format for small-scale GST-tagged protein purification and screening workflows. John Morris Group can support Australian laboratories with Cytiva GST SpinTrap columns for affinity purification, expression screening and protein sample preparation.
