The Whatman Protran BA85 Membrane, 0.45 µm, 25 mm (Part Number 10402578) is a circular nitrocellulose membrane for flow-through and blotting workflows. It is supplied as 25 mm circles in a pack of 1000 and has a 0.45 µm pore size for larger molecular weight samples.
Key Features:
- 0.45 µm BA85 pore structure, intended for blotting applications involving larger molecular weight samples
- 100% pure nitrocellulose membrane, supporting high binding capacity for biomolecule detection
- 25 mm circular format, supplied as 1000 membrane circles per pack
- Protein binding capacity of 75 to 110 µg/cm², supporting protein immobilisation workflows
- Low-background membrane surface, designed to support strong signal-to-noise performance without stringent washing conditions
- Triton-free matrix, suitable for established protein and nucleic acid blotting methods
- Broad detection-method compatibility, including isotopic, chemiluminescent, colorimetric and fluorescent methods
- No methanol pre-wetting step required, as the membrane can be wetted in water before placement into transfer buffer
Benefits:
- Provides a defined membrane option for transfer workflows involving larger molecular weight samples.
- Helps support clearer detection through low-background membrane characteristics.
- Allows laboratories to use one membrane format across multiple established blotting and detection approaches.
- Supports efficient preparation by eliminating a methanol pre-wetting step before transfer.
Why Choose the Whatman Protran BA85 Membrane, 0.45 µm, 25 mm, Part Number 10402578?
This exact Protran BA85 configuration combines a 0.45 µm nitrocellulose membrane with a 25 mm circle format and a high-volume 1000-piece pack. John Morris Group can assist with availability and selection of compatible blotting consumables for your workflow.
