The Amersham ECL Start Western Blotting Detection Reagent, 400 mL (RPN3244) is a luminol-based chemiluminescent substrate for detecting proteins immobilised on Western blot membranes. This 400 mL pack is listed for 4000 cm² of membrane and is recommended for confirmatory analysis and detection of high to medium levels of endogenous proteins.
Key Features
- Luminol-based chemiluminescent detection, designed for protein detection on Western blot membranes
- Recommended for high to medium protein levels, supporting routine confirmatory Western blot analysis
- Long signal duration, retaining usable signal for up to 3 h after working solution addition
- Multiple exposure flexibility, giving laboratories a wider time window between experiment completion and analysis
- Low background performance, producing low background across supported detection methods
- Stable working solution, with the prepared working solution usable for up to 5 d
- 4000 cm² membrane coverage, supplied as a 400 mL pack for larger Western blotting workloads
- X-ray film and CCD imaging compatibility, with chemiluminescent signal emission at 428 nm
Benefits
- Helps laboratories confirm protein presence in routine Western blot workflows.
- Supports flexible imaging schedules when multiple exposures or larger experiment sets are required.
- Provides a practical 400 mL pack size for laboratories processing greater membrane area.
- Works with common chemiluminescent Western blot detection setups using film or CCD imaging.
Why Choose the Amersham ECL Start Western Blotting Detection Reagent, 400 mL (RPN3244)?
Amersham ECL Start RPN3244 provides a reliable entry-level chemiluminescent detection option for high to medium expressed proteins in Western blotting. John Morris Group supplies Amersham blotting standards and reagents to Australian laboratories requiring dependable protein analysis consumables.
