The Amersham ECL Prime Western Blotting Detection Reagent, 3000 cm² (RPN2236) is a chemiluminescent detection reagent system for Western blotting. Supplied for 3000 cm² of membrane, the kit includes Solution A luminol solution and Solution B peroxide solution in 3 × 50 mL bottles each.
Key Features
- High sensitivity Western blot detection, with picogram-level protein detection performance
- Long signal duration, designed to provide signal output for 24 hours
- Stable signal emission, supporting multiple exposures and larger experimental series
- Efficient antibody use, allowing highly diluted primary and secondary antibodies without reducing sensitivity
- 3000 cm² membrane coverage, suitable for higher volume Western blot detection workflows
- Two-solution reagent format, supplied as 3 × 50 mL Solution A and 3 × 50 mL Solution B
- Simple 1:1 working mix, with detection solutions A and B mixed in equal volumes before use
- Defined reagent use rate, with 0.1 mL of working detection reagent required per cm² of membrane
Benefits
- Helps detect low abundance proteins in sensitive Western blot applications.
- Supports repeat imaging and convenient handling time between experiment completion and detection.
- Helps reduce antibody consumption in routine blotting workflows.
- Provides a practical 3000 cm² pack size for laboratories processing larger membrane areas.
- Supports CCD camera detection and autoradiography film workflows.
Why Choose the Amersham ECL Prime Western Blotting Detection Reagent, 3000 cm² (RPN2236)?
Amersham ECL Prime RPN2236 is a high sensitivity ECL Western blotting reagent designed for stable signal output, quantitative detection and efficient antibody use. John Morris Group supplies Amersham blotting standards and reagents to Australian laboratories requiring reliable protein analysis consumables.
