Amersham ECL Select Western Blotting Reagent, 1000 cm² (RPN2235) is a high-sensitivity chemiluminescent detection reagent for Western blotting. The kit contains Solution A luminol solution and Solution B peroxide solution, with 100 mL total reagent volume for 1000 cm² of membrane. It is designed for quantitative analysis of medium to very low abundance proteins.
Key Features
- High-intensity chemiluminescent detection, designed for demanding Western blot sensitivity requirements
- Quantitative analysis support, suited to medium to very low abundance protein detection
- 1000 cm² membrane coverage, supplied as 50 mL luminol solution and 50 mL peroxide solution
- Compatible membrane options, for use with PVDF and nitrocellulose membranes including Amersham Hybond and Amersham Protran ranges
- CCD and film detection flexibility, with output light detectable using CCD imaging systems or X-ray film
- Short signal duration profile, with signal duration stated as less than 2 hours in Cytiva selection guidance
- Blocking reagent compatibility, compatible with Amersham ECL Prime Blocking Agent, Amersham ECL Blocking Agent, BSA and non-fat dry milk
Benefits
- Helps laboratories detect lower abundance proteins where stronger chemiluminescent signal output is required.
- Supports quantitative Western blot workflows for protein confirmation, regulation and expression analysis.
- Provides a practical 1000 cm² format for routine laboratory use without moving to a larger reagent pack.
- Works with common PVDF and nitrocellulose membrane workflows, helping reduce changes to established blotting methods.
- Allows detection by CCD imaging or X-ray film, supporting flexibility across different laboratory imaging setups.
Why Choose the Amersham ECL Select Western Blotting Reagent, 1000 cm² (RPN2235)?
The Amersham ECL Select Western Blotting Reagent, 1000 cm² (RPN2235) is a strong option for Western blot workflows requiring high signal intensity and analysis of medium to very low abundance proteins. John Morris Group supplies Cytiva and Amersham protein analysis reagents across Australia and can help confirm suitability and availability for your laboratory workflow.
