The Amersham ECL Western Blotting Reagent, 1000 cm² (RPN2109) is an enhanced chemiluminescence detection reagent set for routine western blotting. It includes detection reagent 1 and detection reagent 2, each supplied at 62.5 mL, with sufficient volume for 1000 cm² of membrane.
Key Features
- Enhanced luminol-based detection, suitable for routine western blotting applications using chemiluminescence
- Sufficient for 1000 cm² membrane, matching the RPN2109 pack format for confirmatory western blot workflows
- Two-reagent ECL system, supplied with 62.5 mL detection reagent 1 and 62.5 mL detection reagent 2
- Designed for medium to high expression proteins, supporting reliable detection in routine protein blotting applications
- Compatible with film or CCD imaging, with detection possible using Amersham Hyperfilm ECL or a CCD imager
- Higher sensitivity than colourimetric assays, with at least 10-fold greater sensitivity reported for ECL western blotting detection
- Refrigerated storage requirement, with components stored at 2 °C to 8 °C on receipt
Benefits
- Supports routine and confirmatory western blot detection with a widely used chemiluminescent ECL workflow.
- Helps laboratories detect medium to high abundance proteins with stronger sensitivity than colourimetric methods.
- Provides a clearly defined 1000 cm² membrane coverage format for planning blotting reagent use.
- Allows flexible image capture using compatible film-based or CCD-based detection systems.
Why Choose the Amersham ECL Western Blotting Reagent, 1000 cm² (RPN2109)?
The Amersham RPN2109 ECL Western blotting reagent provides a 1000 cm² membrane coverage format for routine chemiluminescent western blot detection. John Morris Group can help Australian laboratories source Amersham blotting and detection reagents for protein analysis workflows.
