The pI Markers Low pI Calibration Kit, pH 3.5 to 6.5 (29443548) is a low-range calibration kit for determining protein isoelectric points in isoelectric focusing workflows. It contains lyophilised mixtures of purified, well-characterised protein markers supplied across the pH 3.5 to 6.5 range.
Key Features
- Low-range pI calibration, covering pH 3.5 to 6.5 for isoelectric focusing applications
- Ten-vial kit format, supplied as 10 vials with 325 µg per vial
- Lyophilised purified protein markers, with well-characterised marker proteins for pH gradient calibration
- Defined marker pI values, including amyloglucosidase, glucose oxidase, soybean trypsin inhibitor, beta-lactoglobulin A and carbonic anhydrase B markers
- Distinct focused bands, helping users calibrate the pH gradient and determine sample protein pI values
- Simple reconstitution, with one vial dissolved in 100 µL of water, or 50 µL when used with PhastGel
- Cold storage format, with unopened kit storage specified at 2°C to 8°C
Note: pI Calibration Kits are not recommended for denaturing conditions such as 8 M urea, as these conditions can alter the protein pattern.
Benefits
- Helps laboratories calibrate pH gradients in IEF gels using protein markers with defined pI values.
- Supports accurate estimation of unknown protein isoelectric points by running markers alongside samples.
- Provides a convenient lyophilised format for routine IEF calibration workflows.
- Allows users to choose a low-range kit when the expected protein pI falls within the acidic to near-neutral range.
Why Choose the pI Markers Low pI Calibration Kit, pH 3.5 to 6.5 (29443548)?
The pI Markers Low pI Calibration Kit, pH 3.5 to 6.5 (29443548) is a practical option for laboratories performing low-range isoelectric focusing and pH gradient calibration. John Morris Group can support Australian laboratories with supply, ordering assistance and product selection for Cytiva electrophoresis consumables.
