The Pharmalyte Carrier Ampholytes, pH 3 to 10, 25 mL (17045601) is a broad range liquid carrier ampholyte reagent for isoelectric focusing. It forms a stable, linear pH gradient with even conductivity across gel or capillary formats, helping separate proteins and peptides by isoelectric point. This 25 mL pack is formulated from co-polymerised glycine, glycylglycine, amines and epichlorohydrin for IEF and IEF-PAGE quality control workflows.
Key Features
- Broad pH 3 to 10 range, supporting wide-range isoelectric focusing of proteins and peptides
- Stable linear pH gradient formation, helping produce reproducible IEF separations
- Even conductivity across the gel or capillary, supporting consistent focusing conditions
- Liquid carrier ampholyte format, supplied as a 25 mL reagent for laboratory IEF workflows
- Co-polymerised ampholyte formulation, made from glycine, glycylglycine, amines and epichlorohydrin
- High buffering capacity per pH unit, helping support high-resolution focusing
- Direct gel compatibility, suitable for agarose, Ultrodex or polyacrylamide gels
- Colourless to light yellow appearance, with storage required at 2°C to 8°C
Benefits
- Helps separate proteins and peptides according to their isoelectric point across a broad pH interval.
- Supports general IEF screening where a wide pH range is required before narrowing the separation window.
- Provides a ready liquid reagent format for gel and capillary focusing applications.
- Supports quality control workflows using IEF and IEF-PAGE methods.
Why Choose the Pharmalyte Carrier Ampholytes, pH 3 to 10, 25 mL (17045601)?
Choose the Pharmalyte Carrier Ampholytes, pH 3 to 10, 25 mL (17045601) when your protein analysis workflow requires a broad range carrier ampholyte for stable pH gradient formation. John Morris Group supplies Pharmalyte and Cytiva electrophoresis reagents for Australian laboratories carrying out IEF, IEF-PAGE and related protein separation workflows.
