Pharmalyte carrier ampholytes, pH 5 to 8, 25 mL (17045301) are narrow range carrier ampholytes for isoelectric focusing and IEF-PAGE quality control. They support separation of charged proteins or peptides by isoelectric point and form a stable linear pH gradient with even conductivity in gel or capillary workflows.
Key Features
- Narrow pH 5 to 8 interval, suited to focused isoelectric focusing work in this pH range
- Stable linear pH gradient formation, supporting even conductivity across gels or capillaries
- High buffering capacity, with Pharmalyte buffering capacity specified at 0.35 mmol/pH/mL
- Co-polymerised ampholyte formulation, made from glycine, glycylglycine, amines and epichlorohydrin
- Liquid reagent format, supplied as a 25 mL pack for laboratory IEF workflows
- Compatible IEF gel use, with recommended use in polyacrylamide, agarose and Ultrodex gel methods
- Controlled storage conditions, specified for storage at 2 °C to 8 °C
Benefits
- Helps separate proteins or peptides according to their isoelectric point in IEF workflows.
- Supports reproducible pH gradient formation for quality control and protein analysis applications.
- Provides a narrow range option where higher resolution is required around pH 5 to 8.
- Can be used across common IEF gel formats, helping laboratories standardise method setup.
Why Choose the Pharmalyte Carrier Ampholytes, pH 5 to 8, 25 mL (17045301)?
Pharmalyte carrier ampholytes, pH 5 to 8, 25 mL (17045301) provide a narrow range reagent option for isoelectric focusing, IEF-PAGE quality control and protein analysis workflows. John Morris Group supplies Pharmalyte reagents for Australian laboratories needing reliable carrier ampholytes for pH gradient separation. Contact John Morris Group to confirm current availability; this item may be succeeded by part 17056601.
