Pharmalyte Carrier Ampholytes pH 4 to 6.5, 25 mL (17045201) is a carrier ampholyte reagent for isoelectric focusing workflows. It is supplied as a 25 mL liquid reagent for forming pH gradients across the pH 4 to 6.5 interval in protein and peptide separation applications.
Key Features
- pH 4 to 6.5 carrier ampholyte range, suited to isoelectric focusing across this defined pH interval
- 25 mL liquid pack, matched to product code 17045201 for clear ordering and replacement control
- High buffering capacity, with Pharmalyte specified at 0.35 mmol per pH unit per mL
- Supports gel and column workflows, with recommended final dilutions of 1:16 in polyacrylamide and agarose gels, 1:15 in Ultrodex gels, and 1:40 in columns
- Stable pH gradient formation, helping create linear gradients for isoelectric focusing applications
- Copolymerised ampholyte formulation, made from glycine, glycylglycine, amines, and epichlorohydrin
Benefits
- Helps separate charged molecules by isoelectric point in IEF and IEF-PAGE quality control workflows.
- Provides a defined pH interval for focused selection when the target pI range is known.
- Supports reproducible method setup with published dilution guidance for common IEF formats.
- Simplifies reagent selection with one matched product code for the pH 4 to 6.5, 25 mL variant.
Why Choose the Pharmalyte Carrier Ampholytes pH 4 to 6.5, 25 mL (17045201)?
Choose Pharmalyte Carrier Ampholytes pH 4 to 6.5, 25 mL (17045201) when you need a defined carrier ampholyte reagent for IEF gradient formation. John Morris Group supplies Cytiva and Pharmalyte reagents for Australian laboratories requiring reliable protein analysis consumables.
