The Amersham Cy5-dUTP Fluorescent Nucleotide, 25 nmol (PA55022) is 5-amino-propargyl-2'-deoxyuridine 5'-triphosphate coupled to Cy5 fluorescent dye for non-radioactive labelling of DNA probes, oligonucleotides and other nucleic acids. It is supplied as 25 nmol Cy5-dUTP in 10 mM phosphate buffer at pH 7.0 and provides far-red Cy5 fluorescence for applications such as microarray detection, FISH and other nucleic acid probe workflows.
Key Features
- Cy5-dUTP fluorescent nucleotide, based on Cy5-AP3-dUTP for incorporation into nucleic acid labelling workflows
- 25 nmol reagent format, supplied as Cy5-dUTP in 10 mM phosphate buffer at pH 7.0
- Far-red Cy5 fluorescence, with absorbance maximum at 649 nm and emission maximum at 670 nm
- High sensitivity fluorescent signal, supporting detection where meaningful data from low copy number transcripts is required
- Spectrally resolved dye performance, helping support multicolour analysis within a single sample
- Low nonspecific binding and high photostability, supporting cleaner fluorescent detection workflows
- Water-soluble and pH-insensitive CyDye chemistry, designed for fluorescence analysis in genomic and protein research
- Frozen dark storage, specified at -15°C to -30°C in the dark, with protection from light during handling
Benefits
- Supports non-radioactive labelling of DNA and RNA probes for fluorescence-based analysis.
- Helps enable multicolour workflows in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping and gene mapping.
- Provides far-red Cy5 fluorescence with narrow emission characteristics for clear spectral separation in multiplexed experiments.
- Pairs well with Cy3-labelled nucleotides in dual-colour microarray and probe detection workflows.
- Cold, light-protected handling helps maintain reagent performance across sensitive labelling workflows.
Why Choose the Amersham Cy5-dUTP Fluorescent Nucleotide, 25 nmol (PA55022)?
Choose the Amersham Cy5-dUTP Fluorescent Nucleotide, 25 nmol (PA55022) when your laboratory needs a Cy5-labelled nucleotide for non-radioactive probe labelling and far-red fluorescence detection. John Morris Group supplies Cytiva and Amersham fluorescent labelling reagents to Australian laboratories, helping you source the correct nucleotide, pack size and supporting documentation for your workflow.
