GenomiPhi Single Cell DNA Kit, 25 Reactions (29108107) is a whole genome DNA amplification kit for genomic DNA amplification from 1 to 1000 cells. It uses Phi29 DNA polymerase based multiple displacement amplification and is supplied for 25 reactions.
Key Features
- Single cell genomic DNA amplification, formulated to amplify genomic DNA from 1 to 1000 cells
- Phi29 DNA polymerase chemistry, using multiple displacement amplification for isothermal whole genome amplification
- Two-step workflow, designed to generate amplified genomic DNA for downstream applications
- Low input sensitivity, with reported amplification sensitivity down to 1 fg of genomic DNA
- Typical microgram DNA yield, with typical yields of 4 to 7 µg of genomic DNA reported for the kit
- Fast workflow, with amplification workflows completed in less than 3 hours and less than 15 minutes of hands-on time
- Downstream application support, with use reported for SNP analysis, array comparative genomic hybridisation and next-generation sequencing
- Pack of 25 reactions, supplied as the 25-reaction GenomiPhi Single Cell DNA Amplification Kit
Benefits
- Helps generate sufficient genomic DNA from very small single cell or low cell number samples.
- Supports single cell genomics workflows where coverage, amplification uniformity and low background are important.
- Provides a practical amplification step before downstream genomic analysis methods.
- Reduces manual handling time with a simple workflow for routine molecular biology laboratories.
Why Choose the GenomiPhi Single Cell DNA Kit, 25 Reactions (29108107)?
GenomiPhi Single Cell DNA Kit, 25 Reactions (29108107) is suited to laboratories needing whole genome amplification from single cells or very low input genomic DNA samples. John Morris Group supplies Cytiva molecular biology consumables across Australia with support for product selection and ordering.
