GenomiPhi Single Cell DNA Amplification Kit, 100 Reactions (29108039) is a whole genome amplification kit for amplifying genomic DNA from very small cell inputs. It uses Phi29 DNA polymerase based multiple displacement amplification to generate microgram quantities of genomic DNA while suppressing background amplification.
Key Features
- Single-cell genomic DNA amplification, optimised to amplify genomic DNA from as little as one cell
- 100 reaction kit format, matching product code 29108039 for the 100 reaction pack
- Phi29 polymerase technology, using multiple displacement amplification for non-PCR whole genome amplification
- Two-step workflow, designed to amplify genomic DNA from very small starting material with minimal process complexity
- 1 to 1000 cell input range, supporting uniform genomic DNA amplification across low cell numbers
- Typical 4 to 7 µg DNA yield, providing microgram quantities of genomic DNA for downstream analysis
- Suppressed background amplification, helping ensure that input DNA is preferentially amplified during incubation
- Downstream application support, with amplified DNA used in aCGH, SNP analysis, and next-generation sequencing
Benefits
- Helps generate usable genomic DNA quantities from limited or single-cell samples.
- Supports sensitive workflows where background amplification needs to be minimised.
- Provides a practical 100 reaction format for laboratories running repeated single-cell amplification work.
- Supports downstream genomic analysis where amplified DNA is required from very low input material.
Why Choose the GenomiPhi Single Cell DNA Amplification Kit, 100 Reactions (29108039)?
GenomiPhi Single Cell DNA Amplification Kit, 100 Reactions (29108039) is suitable for laboratories needing whole genome amplification from low cell numbers or single-cell samples. John Morris Group supplies molecular and immunodiagnostic consumables for Australian laboratories working across genomic DNA preparation and analysis.
