The TempliPhi Sequence Resolver Kit, 200 Reactions (28903531) uses modified-nucleotide rolling circle amplification to prepare circular DNA templates for sequencing. It is designed to improve sequencing results from difficult templates, including those affected by secondary structures, high GC content, repeats, sequencing stops, and compression issues.
Key Features:
- Modified-nucleotide rolling circle amplification, uses Phi29 DNA polymerase to amplify circular DNA templates for subsequent sequencing
- Designed for difficult sequencing templates, supports resolution of common issues including sequence stops, compressions, repeats, secondary structures, and GC-rich regions
- Culture-free template preparation, supports DNA preparation from bacterial colonies, glycerol stocks, or purified DNA
- Direct cycle-sequencing use, prepared DNA can be used in cycle sequencing reactions without a separate purification step
- Strong difficult-template read performance, supports up to 820 bp Phred 20 read length for challenging sequencing templates
- Practical 200-reaction pack, provides capacity for routine sequence-finishing and template-preparation workflows
- Complete reagent set, supplied with sample buffer, reaction buffer, and enzyme mix
Benefits
- Improve the likelihood of obtaining usable sequencing data from challenging circular DNA templates.
- Reduce workflow steps by preparing DNA directly from colony, glycerol-stock, or purified-DNA starting material.
- Avoid separate DNA purification before the cycle sequencing reaction.
- Help reduce reliance on dGTP chemistry and sequencing-reaction additives for difficult-template sequencing.
Why Choose the TempliPhi Sequence Resolver Kit, 200 Reactions (28903531)?
The TempliPhi Sequence Resolver Kit, 200 Reactions (28903531) provides a dedicated solution for preparing difficult circular DNA templates before sequencing. Its modified-nucleotide rolling circle amplification workflow supports sequence finishing where conventional approaches may leave gaps or compression-related issues. John Morris Group can assist with ordering and product selection.
