The GenomiPhi V2 DNA Amplification Kit, 500 Reactions (25660032) is a whole genome amplification kit for generating amplified DNA from nanogram amounts of starting material. It uses Phi29-based isothermal strand displacement amplification and is supplied in a 500 reaction format for smaller-scale whole genome amplification workflows.
Key Features
- 500 reaction kit format, matching the verified pack size for product code 25660032
- Phi29 isothermal amplification method, using strand displacement amplification without requiring a thermocycler
- Typical 4 to 7 µg DNA yield, generated from nanogram amounts of DNA sample in less than 2 hours
- Average product length over 10 kb, supporting downstream genomic analysis applications
- Representative whole genome amplification, helping maintain locus representation close to the original DNA sample
- High-fidelity amplification chemistry, supported by Phi29 DNA polymerase proofreading activity
- Flexible starting material compatibility, suitable for purified DNA or nonpurified cell lysate workflows
- Complete kit components included, with sample buffer, reaction buffer, enzyme mix and lambda control DNA
Note: Store the kit at -70°C. The enzyme mix must be stored at -70°C, while the other components may be stored at -20°C.
Benefits
- Helps amplify limited genomic DNA input into microgram quantities for downstream analysis.
- Supports faster setup by providing the required whole genome amplification components in one kit.
- Removes the need for thermocycling during amplification, simplifying workflow setup.
- Useful for downstream applications such as genotyping, array CGH, cloning, sequencing and DNA archival.
- Supports multiple DNA preparation workflows, including purified DNA samples and suitable cell lysate inputs.
Why Choose the GenomiPhi V2 DNA Amplification Kit, 500 Reactions (25660032)?
The GenomiPhi V2 DNA Amplification Kit, 500 Reactions (25660032) provides a verified 500 reaction format for Phi29-based whole genome amplification, with typical 4 to 7 µg DNA yield and product lengths over 10 kb. John Morris Group can support Australian laboratories with GenomiPhi DNA amplification kits and related molecular biology consumables.
