The TriplePrep Kit, 50 Purifications (28942544) is an extraction kit for rapid, simultaneous isolation of genomic DNA, total RNA and total denatured proteins from animal tissues and mammalian cells. It is supplied for 50 purifications and supports workflows where gene, transcript and protein data need to be generated from the same sample.
Key Features
- Three analytes from one sample, allowing genomic DNA, total RNA and total denatured proteins to be isolated from undivided samples
- 50 purification kit format, supplied with a protocol and components sufficient for 50 preparations
- Animal tissue and mammalian cell compatibility, with stated sample processing ranges of 1 to 20 mg rat liver or 3 x 10^5 to 5 x 10^6 HeLa cells
- Rapid workflow, with a stated working time of 45 to 60 minutes, excluding lysis time
- High-quality genomic DNA output, with stated gDNA purity of ≥1.7 and gDNA size of ≥15 kb
- High-quality total RNA output, with stated total RNA purity of ≥1.9
- DNase I included, supporting RNA preparation within the kit workflow
- Colour-coded workflow components, with caps and bottles matched to protocol steps to help reduce handling errors
Benefits
- Helps researchers correlate genomic, transcriptomic and protein data from the same limited sample.
- Reduces the need to split precious animal tissue or mammalian cell samples across separate extraction workflows.
- Supports downstream applications including PCR, sequencing, RT-qPCR, gene expression microarray, SDS-PAGE, Western blotting, 2-D DIGE and LCMS.
- Allows isolation of any two or all three analytes depending on the experimental design.
- Provides a streamlined kit format for biomarker, gene expression and protein expression studies.
Why Choose the TriplePrep Kit, 50 Purifications (28942544)?
The TriplePrep Kit, 50 Purifications (28942544) is suited to laboratories that need to maximise information from the same biological sample. John Morris Group can support Australian laboratories with Cytiva extraction kits for molecular biology, genomics, transcriptomics and protein analysis workflows.
