The Blood genomicPrep Mini Spin Kit, 250 Purifications (28904265) is designed for rapid extraction and purification of high molecular weight genomic DNA from whole blood, Buffy coat, bone marrow cells, and nucleated blood. It uses a silica-membrane mini spin-column workflow to produce genomic DNA with a characteristic size greater than 20 kbp for downstream molecular biology applications.
Key Features:
- Broad blood-derived sample compatibility, suitable for whole blood, Buffy coat, bone marrow cells, and nucleated blood
- Flexible sample-volume processing, handles 50 to 300 µL of whole blood or cell fractions by direct lysis, with a two-stage lysis method for 300 to 1000 µL whole-blood samples
- Selective silica-membrane purification, uses chaotropic lysis and Proteinase K to promote genomic DNA binding while contaminants are removed during washing
- High molecular weight genomic DNA, produces DNA with a characteristic size greater than 20 kbp using a protocol designed to minimise shearing
- Typical DNA yield of 4 to 6 µg, from 200 µL of whole blood, with an A260/A280 purity ratio greater than 1.7
- Efficient five-step workflow, completes in less than 20 minutes per preparation under the stated protocol conditions
- Complete 250-purification kit, supplied with mini spin columns, Proteinase K, lysis, wash and elution buffers, collection tubes, protocol booklet, and quick reference card
Benefits
- Prepare purified genomic DNA for cloning, restriction enzyme digestion, PCR amplification, and genotyping workflows.
- Process common blood-derived sample types with a defined genomic DNA extraction method.
- Scale whole-blood input from standard sample volumes up to 1 mL using the appropriate supplied protocol.
- Support routine laboratory throughput with a 250-purification pack format.
Why Choose the Blood genomicPrep Mini Spin Kit, 250 Purifications (28904265)?
The Blood genomicPrep Mini Spin Kit, 250 Purifications (28904265) provides a defined silica-membrane method for laboratories purifying genomic DNA from whole blood and related cell fractions. It combines high molecular weight DNA recovery, flexible input handling, and a practical 250-purification format. John Morris Group can assist with product selection and ordering.
