Blood genomicPrep Mini Spin Kit, 50 Purifications (28904264), is a silica-membrane spin-column kit for rapid extraction and purification of high-molecular-weight genomic DNA from whole blood, buffy coat, bone marrow cells and nucleated blood. It supports 50 to 1,000 µL whole-blood samples and is supplied for 50 purifications.
Key Features:
- Designed for blood-derived samples, supporting whole blood, buffy coat, bone marrow cells and nucleated blood
- Flexible sample-volume handling, processing 50 to 300 µL whole blood, buffy coat or bone marrow cells, 2 to 10 µL nucleated blood, and up to 1,000 µL whole blood with the larger-volume protocol
- Proteinase K and chaotropic lysis chemistry, releasing genomic DNA while denaturing protein components
- Selective silica-membrane binding, allowing genomic DNA to bind while denatured contaminants are removed through wash steps
- High-molecular-weight genomic DNA, producing DNA greater than 20 kbp, with 90% of purified product greater than 20 kbp
- Defined yield and purity performance, delivering 4 to 6 µg genomic DNA from 200 µL whole blood with an A260/A280 ratio greater than 1.7
- Five-step purification workflow, completing lysis, binding, washing, drying and elution in less than 20 minutes
- Consistent 200 µL elution format, with a maximum binding capacity of 60 µg genomic DNA
Benefits
- Helps prepare purified genomic DNA for cloning, restriction enzyme digestion, PCR amplification and genotyping workflows.
- Supports common blood and blood-cell sample types using one defined purification kit.
- Provides a rapid workflow for laboratories requiring intact genomic DNA from blood-derived samples.
- Helps remove proteins, salts and other contaminants before downstream molecular biology applications.
Why Choose the Blood genomicPrep Mini Spin Kit, 50 Purifications (28904264)?
Blood genomicPrep Mini Spin Kit, 50 Purifications (28904264), combines Proteinase K lysis with silica-membrane purification for extraction of high-molecular-weight genomic DNA from a range of blood-derived samples. The kit provides defined sample input options and a rapid, five-step workflow. John Morris Group can assist with product selection and ordering for part 28904264.
