Bacteria genomicPrep Mini Spin Kit, 50 Purifications (28904258), is designed for rapid extraction and purification of genomic DNA from Gram-negative and Gram-positive bacteria. The kit combines proteinase K lysis with silica spin-column purification to produce genomic DNA larger than 20 kb for downstream molecular biology workflows.
Key Features:
- Dedicated bacterial DNA extraction workflow, with separate protocols for Gram-negative and Gram-positive bacteria
- Silica spin-column purification, using chaotropic conditions to bind genomic DNA while contaminants are removed during wash steps
- High-molecular-weight genomic DNA, producing DNA greater than 20 kb with protocols designed to minimise shearing
- Defined bacterial cell input, processing 1 to 4 × 109 cells per preparation
- Typical genomic DNA yields, delivering 4 to 12 µg from Gram-negative bacteria and 5 to 10 µg from Gram-positive bacteria, based on 2 × 109 cells
- 200 µL elution volume, providing a consistent final format for purified genomic DNA
- Rapid preparation times, with approximately 40 minutes for Gram-negative bacteria and 60 minutes for Gram-positive bacteria, excluding optional RNase treatment
- Optional RNA removal, allowing RNA-free genomic DNA preparation when an RNase step is included
- Colour-coded kit components, helping users follow the protocol steps and reduce handling errors
Note: This kit is not suitable for RNA purification.
Benefits
- Supports preparation of bacterial genomic DNA for PCR, cloning, restriction enzyme digestion and genotyping.
- Provides tailored workflows for both Gram-negative and Gram-positive bacterial samples.
- Helps produce intact, high-purity genomic DNA for molecular biology applications.
- Reduces handling complexity through a streamlined spin-column process and colour-coded components.
Why Choose the Bacteria genomicPrep Mini Spin Kit, 50 Purifications (28904258)?
Bacteria genomicPrep Mini Spin Kit, 50 Purifications (28904258), provides a practical workflow for laboratories extracting high-molecular-weight genomic DNA from bacterial cultures. Its dedicated Gram-negative and Gram-positive protocols support reliable purification across common bacterial sample types. John Morris Group can assist with product selection and ordering for part 28904258.
