The AutoSeq G-50 Dye Terminator Removal Columns, 50 Pack (27534001) are ready-to-use spin columns for removing unincorporated fluorescent dye-terminators from DNA cycle sequencing reactions before automated sequencing. Each column is prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide as a preservative.
Key Features
- Designed for dye-terminator removal, supporting cleanup of DNA cycle sequencing reactions before automated sequencing
- Prepacked Sephadex G-50 DNA Grade F media, supplied in ready-to-use MicroSpin column format
- Pre-equilibrated in double-distilled water, helping avoid salt-related artefacts on salt-sensitive automated DNA sequencers
- 0.05% Kathon CG/ICP Biocide preservative, included in the pre-equilibrated columns
- Less than 4 minute workflow, enabling sample application through to purified product collection in under 4 minutes
- Typical 12 to 25 µL sample volume, matching the verified application guidance
- Suitable for DNA greater than 20 bases, based on the verified product listing
- 50 column pack size, matching the 27534001 product variant
Benefits
- Helps improve automated sequencing quality by removing residual dye-terminators that can obscure data.
- Saves time compared with slower cleanup methods by using ready-to-use prepacked columns.
- Supports direct purification of cycle sequencing reactions without requiring sample dilution.
- Provides a convenient 50 column pack for labs running smaller sequencing cleanup batches.
Why Choose the AutoSeq G-50 Dye Terminator Removal Columns, 50 Pack (27534001)?
The AutoSeq G-50 Dye Terminator Removal Columns, 50 Pack (27534001) are a verified Cytiva variant for laboratories needing rapid cleanup of fluorescent dye-terminators before automated DNA sequencing. John Morris Group supplies molecular biology and sequencing cleanup consumables across Australia with local support for product selection and ordering.
