The NICK Columns, 20 Pack (17085501) are disposable gravity-flow columns for purifying nick-translated DNA fragments and separating labelled probes from unincorporated labelled nucleotides. They are prepacked with Sephadex G-50 DNA Grade and accommodate 1 to 100 µL input sample volumes for rapid gel filtration workflows.
Key Features
- Designed for labelled DNA purification, suitable for oligonucleotides and small DNA fragments greater than 20 bases in length
- Gravity-flow chromatography format, allowing ready-to-use purification without a pump or centrifugation step
- Prepacked Sephadex G-50 DNA Grade matrix, supplied in distilled water with 0.15% Kathon CG/ICP Biocide as preservative
- 1 to 100 µL input sample volume, suitable for small-volume DNA labelling reaction clean up
- High DNA recovery, with verified yield or recovery greater than 90%
- Low recovered salt contamination, typically less than 0.2%
- 100 µg column capacity, with guidance not to load DNA solutions above 1 mg/mL
- Pack of 20 columns, suitable for routine molecular biology purification workflows
Benefits
- Helps separate labelled DNA from unincorporated labelled nucleotides after DNA labelling reactions.
- Provides a simple disposable format for routine probe purification and hybridisation workflows.
- Supports small-volume sample clean up with minimal setup and gravity-flow handling.
- Helps preserve purified DNA for downstream applications without removing or denaturing enzyme.
Why Choose the NICK Columns, 20 Pack (17085501)?
Choose the NICK Columns, 20 Pack (17085501) when you need a ready-to-use gravity-flow column for purifying labelled DNA greater than 20 bases in length from unincorporated labelled nucleotides. John Morris Group supplies Cytiva molecular biology consumables for Australian laboratories requiring reliable nucleic acid clean up products.
