The Amersham NAP-5 DNA Purification Columns, Pack of 50 (17085302), are disposable size exclusion columns prepacked with Sephadex G-25 DNA Grade resin for gravity-flow purification of nucleic acids. They are designed for oligonucleotides and small DNA fragments, desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelling reactions. This NAP-5 pack provides 50 columns with a maximum input sample volume of 0.5 mL.
Key Features
- Gravity-flow purification, requiring no pump or centrifuge for standard column operation
- Sephadex G-25 DNA Grade matrix, prepacked for gel filtration of nucleic acids
- NAP-5 format for small samples, with a maximum input sample volume of 0.5 mL
- Suitable for short nucleic acids, recovering labelled DNA greater than 10 bp with no stated maximum oligonucleotide length
- High DNA recovery, with yield or recovery of DNA greater than 90%
- Low residual salt, with recovered DNA purity typically less than 3% salt contamination
- Ready pack of 50 columns, supporting 50 purification workflows
Benefits
- Simplifies small-scale DNA purification, desalting and buffer exchange using gravity flow.
- Helps prepare oligonucleotides and small DNA fragments for PCR, sequencing and labelling workflows.
- Reduces setup time with prepacked disposable columns and a defined NAP-5 sample volume range.
- Supports consistent purification performance without users needing to pack columns manually.
- Provides a practical 50-column pack for routine nucleic acid clean-up work in molecular biology labs.
Why Choose the Amersham NAP-5 DNA Purification Columns, Pack of 50 (17085302)?
Choose the Amersham NAP-5 DNA Purification Columns when you need gravity-flow clean-up of oligonucleotides or small DNA fragments from salts, buffers or unincorporated nucleotides. With verified NAP-5 specifications including 0.5 mL maximum input sample volume, greater than 90% DNA recovery and a 50-column pack, this product suits routine PCR clean-up and size selection workflows. John Morris Group supplies Cytiva and Amersham nucleic acid purification consumables for Australian laboratories.
