NAP-5 DNA Purification Columns, 20 Columns (17085301) are disposable gravity flow columns for purification of oligonucleotides and small DNA fragments. The columns are prepacked with Sephadex G-25 DNA Grade resin and support desalting, buffer exchange and removal of unincorporated nucleotides from end-labelling reactions.
Key Features
- Gravity flow purification, allowing DNA clean-up without pumps or centrifugation
- Prepacked Sephadex G-25 DNA Grade matrix, supplied ready for gel filtration workflows
- Suitable for small DNA and oligonucleotides, with recovery of labelled DNA greater than 10 bp
- Up to 0.5 mL input sample volume, matching the NAP-5 format for small-volume purification
- High DNA recovery, with greater than 90% yield or recovery of DNA
- Low salt carryover, with recovered DNA typically showing less than 3% salt contamination
- Pack of 20 columns, supplied for 20 purifications
Benefits
- Supports rapid clean-up of oligonucleotides and small DNA fragments after synthesis or labelling reactions.
- Helps remove salts, exchange buffers and clear unincorporated nucleotides before downstream work.
- Provides a simple gravity-based workflow for PCR, sequencing and labelling applications.
- Reduces setup complexity by using disposable, prepacked columns.
Why Choose the NAP-5 DNA Purification Columns, 20 Columns (17085301)?
NAP-5 DNA Purification Columns provide a practical small-volume format for nucleic acid purification using Sephadex G-25 DNA Grade gel filtration. John Morris Group supplies Cytiva and Amersham laboratory consumables to Australian laboratories with local product support and ordering assistance.
