Sephadex G-25 DNA Grade SF Resin, 100 g (17057202) is a superfine size exclusion chromatography resin for purifying DNA fragments greater than 10 bases in length from small molecules such as salts. It is supplied in dry bulk format so laboratories can prepare their own columns for nucleic acid purification.
Key Features
- DNA Grade SF Sephadex G-25 format, designed for preparing columns for nucleic acid purification
- Suitable for DNA fragments greater than 10 bases, with larger DNA fragments excluded from the resin and passing through without retention
- Dry particle size of 20 to 80 µm, matched to the superfine G-25 DNA Grade format
- pH stability from 2 to 13, supporting use across a broad range of aqueous purification conditions
- Fractionation range of Mr 1000 to 5000, specified for globular proteins as a reference size exclusion range
- Dextran fractionation range of Mp 100 to 5000, supporting gel filtration separation guidance
- High DNA recovery performance, tested to ensure reproducibility and greater than 90% DNA recovery
- 100 g dry bulk pack, allowing researchers to prepare their own columns for DNA and oligonucleotide purification
Benefits
- Helps remove small molecules such as salts from DNA fragments by size exclusion.
- Supports purification of oligonucleotides and small DNA fragments after synthesis or labelling applications.
- Gives laboratories flexibility to prepare their own columns rather than relying only on prepacked formats.
- Provides clear preparation guidance, including swelling time and approximate bed volume, for column packing workflows.
- Supports consistent DNA purification setup with tested recovery and defined resin specifications.
Why Choose the Sephadex G-25 DNA Grade SF Resin, 100 g (17057202)?
Choose Sephadex G-25 DNA Grade SF Resin, 100 g (17057202) when you need a dry bulk resin for preparing DNA purification columns. John Morris Group supplies Cytiva Sephadex resins for Australian laboratories working across molecular biology, nucleic acid purification and small-fragment DNA cleanup workflows.
