Thermo Sequenase DNA Polymerase with TAP, 10000 U (E79000Z) is a thermostable DNA polymerase supplied with Thermoplasma acidophilum inorganic pyrophosphatase for DNA sequencing workflows. The enzyme uses ddNTPs as readily as dNTP substrates at temperatures similar to standard PCR, supporting Sanger sequencing and primer extension applications.
Key Features
- 10000 U enzyme pack, supplied for DNA sequencing and molecular biology workflows
- Thermostable DNA polymerase, designed to operate at temperatures similar to those used in standard PCR
- Efficient ddNTP incorporation, using dideoxynucleotide triphosphates as readily as dNTP substrates
- Supplied with TAP, including Thermoplasma acidophilum inorganic pyrophosphatase in the product format
- Sanger sequencing support, using ddNTP-mediated chain termination for DNA sequencing workflows
- Cycle sequencing suitability, supporting use where limited template quantity may restrict T7 Sequenase DNA polymerase workflows
- Read-through performance, supporting difficult sequence regions such as GC-rich templates at elevated sequencing temperatures
- Frozen storage requirement, listed for storage at -20°C and shipment on dry ice
Benefits
- Supports Sanger and dideoxy sequencing workflows where efficient ddNTP incorporation is required.
- Helps generate uniform, easy-to-read band patterns in sequencing applications.
- Thermostable performance supports sequencing through challenging template structures.
- The 10000 U pack size is suitable for laboratories performing repeated DNA sequencing enzyme workflows.
Why Choose the Thermo Sequenase DNA Polymerase with TAP, 10000 U (E79000Z)?
Thermo Sequenase DNA Polymerase with TAP, 10000 U (E79000Z) provides a verified Cytiva enzyme option for Sanger sequencing, cycle sequencing and primer extension workflows. John Morris Group supplies DNA modifying enzymes to support molecular and immunodiagnostic laboratories across Australia.
