The Thermo Sequenase DNA Polymerase, 1000 Units (E79000Y) is a thermostable DNA polymerase supplied with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP) for DNA sequencing workflows. It uses dideoxynucleotide triphosphates as readily as deoxynucleotide substrates at temperatures similar to standard PCR, supporting cycle sequencing and difficult sequence read-through.
Key Features
- Thermostable sequencing polymerase, designed for DNA sequencing at elevated temperatures similar to standard PCR
- Efficient ddNTP incorporation, using dideoxynucleotide triphosphates as readily as dNTP substrates
- Thermo Sequenase/TAP formulation, supplied with Thermoplasma acidophilum Inorganic Pyrophosphatase
- 1000 unit pack size, matching the verified E79000Y product variant
- 32 units/µL activity, providing a defined enzyme concentration for sequencing reaction setup
- Reaction and dilution buffers included, supplied with Thermo Sequenase/TAP, dilution buffer and reaction buffer
- Difficult sequence read-through support, helping with GC-rich or secondary-structure-prone templates during sequencing
- Cold storage requirement, specified for storage at -20°C in stabilising solution
Benefits
- Supports cycle sequencing workflows where thermostable enzyme performance is required.
- Helps produce uniform, easy-to-read band patterns in sequencing applications.
- Improves ddNTP incorporation to help reduce sequencing issues caused by secondary structure.
- Provides matched enzyme, dilution buffer and reaction buffer components for assay setup.
- Suitable for DNA sequencing and SNP genotyping applications where primer extension is used.
Why Choose the Thermo Sequenase DNA Polymerase, 1000 Units (E79000Y)?
The Thermo Sequenase DNA Polymerase, 1000 Units (E79000Y) is suited to laboratories that need a thermostable sequencing polymerase with TAP for cycle sequencing and primer extension workflows. John Morris Group can support Australian laboratories with sourcing Cytiva DNA modifying enzymes and molecular biology reagents for routine genomics applications.
