Amersham Shrimp Alkaline Phosphatase, 1000 U (E70092Z) is a DNA modifying enzyme for dephosphorylation of DNA, RNA, nucleotides and proteins. It is suitable for dephosphorylation of 5'- and 3'-overhangs, recessed ends, blunt ends and single nucleotides, and can be heat inactivated after incubation.
Key Features
- 1000 U enzyme pack, supplied at an activity of 1 unit/µL for molecular biology workflows
- Heat-inactivated after incubation, listed as inactivated by heating at 75°C for 5 minutes
- DNA and RNA dephosphorylation, catalysing removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides and proteins
- Suitable for multiple DNA end types, including 5'- and 3'-overhangs, recessed ends, blunt ends and single nucleotides
- Active in PCR and restriction endonuclease buffers, supporting common molecular biology reaction workflows
- PCR clean-up support, used together with Exonuclease I for cleaning up PCR reactions before downstream applications such as DNA sequencing
- Recombinant E. coli expression, listed as bacterial alkaline phosphatase recombinantly expressed in E. coli
- Supplied with buffers, including 10× reaction buffer and dilution buffer
Benefits
- Supports dephosphorylation steps before downstream labelling, cloning and sequencing workflows.
- Heat inactivation simplifies post-reaction handling after incubation.
- The 1 unit/µL activity format supports controlled enzyme addition in DNA modification workflows.
- The 1000 U pack size is practical for laboratories performing repeated phosphatase reactions.
Why Choose the Amersham Shrimp Alkaline Phosphatase, 1000 U (E70092Z)?
Amersham Shrimp Alkaline Phosphatase, 1000 U (E70092Z) provides a verified Cytiva enzyme option for dephosphorylation and PCR clean-up applications. John Morris Group supplies DNA modifying enzymes to support molecular and immunodiagnostic workflows across Australian laboratories.
