The Amersham Shrimp Alkaline Phosphatase, 500 Units (E70092Y) is a DNA modifying enzyme for dephosphorylation of nucleic acid substrates. It catalyses the removal of 5' and 3' phosphate groups from DNA, RNA, nucleotides and proteins, and can be heat inactivated after incubation.
Key Features
- Alkaline phosphatase enzyme, used for removal of 5' and 3' phosphate groups from DNA, RNA, nucleotides and proteins
- Broad dephosphorylation scope, suitable for 5' and 3' overhangs, recessed ends, blunt ends and single nucleotides
- 500 unit pack size, matching the verified E70092Y product variant
- 1 unit/µL activity, supplied at a defined activity for controlled reaction setup
- Reaction and dilution buffers included, supplied with 10x reaction buffer and dilution buffer
- Purified enzyme preparation, described as free of contaminating endonucleases, exonucleases and ribonucleases
- Heat inactivation option, inactivated by heating at 75°C for 5 minutes
Benefits
- Supports dephosphorylation workflows before downstream molecular biology applications.
- Helps simplify post-reaction handling because the enzyme can be inactivated by heating.
- Provides matched buffer components for setting up standard reactions.
- Useful for laboratories preparing DNA or RNA substrates where phosphate removal is required.
Why Choose the Amersham Shrimp Alkaline Phosphatase, 500 Units (E70092Y)?
The Amersham Shrimp Alkaline Phosphatase, 500 Units (E70092Y) is suited to laboratories needing a heat-inactivated phosphatase for nucleic acid dephosphorylation workflows. John Morris Group can support Australian laboratories with sourcing Cytiva and Amersham DNA modifying enzymes for routine molecular biology applications.
