Amersham Shrimp Alkaline Phosphatase, 5000 U (E70092X) is a heat-labile alkaline phosphatase for DNA and RNA dephosphorylation workflows. It catalyses removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides and proteins, and can be used for PCR clean-up prior to downstream applications such as sequencing.
Key Features
- 5000 U enzyme pack, supplied for molecular biology and DNA modification workflows
- Heat-labile alkaline phosphatase, designed for easy heat inactivation after incubation
- DNA and RNA dephosphorylation, removing phosphate groups from DNA, RNA, nucleotides and proteins
- PCR clean-up application, used to degrade remaining dNTPs from amplified samples after PCR
- Useful for cloning and sequencing preparation, supporting downstream labelling, cloning, sequencing and SNP analysis workflows
- Supplied with reaction and dilution buffers, with 10× reaction buffer and SAP dilution buffer listed for the product family
- Purified enzyme preparation, listed as purified to apparent homogeneity and free of contaminating endonucleases, exonucleases and ribonucleases
- Frozen storage requirement, listed for storage at -20°C and shipment on dry ice
Benefits
- Helps prepare PCR products for downstream workflows by degrading excess dNTPs after amplification.
- Supports dephosphorylation of DNA and RNA ends for labelling, cloning and sequencing applications.
- Heat inactivation simplifies post-reaction handling without requiring additional removal steps.
- The 5000 U pack size is practical for laboratories performing repeated DNA modification workflows.
Why Choose the Amersham Shrimp Alkaline Phosphatase, 5000 U (E70092X)?
Amersham Shrimp Alkaline Phosphatase, 5000 U (E70092X) provides a verified Cytiva enzyme option for dephosphorylation and PCR clean-up applications. John Morris Group supplies DNA modifying enzymes to support molecular and immunodiagnostic workflows across Australian laboratories.
