The Exonuclease I, 2500 Units (E70073Z) is a DNA modifying enzyme used to selectively digest excess single-stranded DNA in mixtures that also contain double-stranded DNA. It catalyses sequential removal of nucleotides from the 3' end of single-stranded DNA and is commonly used for removing unused primers after PCR.
Key Features
- Selective single-stranded DNA digestion, used to digest excess ssDNA while leaving double-stranded DNA intact
- 3' to 5' exonuclease activity, catalysing sequential nucleotide removal from the 3' end of single-stranded DNA molecules
- PCR cleanup support, suitable for removing unused primers after PCR before downstream use
- 2500 unit pack size, matching the verified E70073Z product variant
- Recombinant bacterial enzyme, described as a monomeric 54.5 kDa bacterial Exonuclease I expressed in E. coli
- Active in PCR buffers, supporting direct use in PCR cleanup workflows
- Heat inactivation option, inactivated by heating at 80°C for 15 minutes
Benefits
- Helps prepare PCR products for downstream workflows by removing residual primers.
- Supports selective cleanup where single-stranded DNA must be digested without degrading double-stranded DNA.
- Can be paired with alkaline phosphatase workflows for PCR reaction cleanup before sequencing or further DNA analysis.
- Heat inactivation helps simplify workflow handling after digestion.
Why Choose the Exonuclease I, 2500 Units (E70073Z)?
The Exonuclease I, 2500 Units (E70073Z) is suited to laboratories needing a selective enzyme for single-stranded DNA digestion and PCR primer cleanup. John Morris Group can support Australian laboratories with sourcing Cytiva DNA modifying enzymes and molecular biology reagents for routine nucleic acid workflows.
