Exonuclease I, 10 U/µL, 5000 Units (E70073X) is a DNA modifying enzyme for selectively digesting single-stranded DNA in mixtures that may also contain double-stranded DNA. It is commonly used to remove unused primers after PCR and catalyses sequential nucleotide removal from the 3' end of single-stranded DNA molecules.
Key Features
- 10 U/µL Exonuclease I format, supplied as a 5000 units pack for molecular biology workflows
- Single-stranded DNA specificity, degrading ssDNA while supporting clean-up in mixtures containing double-stranded DNA
- 3' to 5' exonuclease activity, catalysing sequential nucleotide removal from the 3' end of single-stranded DNA
- PCR clean-up application, used for removal of unused primers after PCR
- E. coli enzyme source, listed as Exonuclease I from Escherichia coli
- Mg2+ ion dependent activity, listed for the E. coli Exonuclease I enzyme
- Heat inactivation option, listed as inactivated by heating at 80°C for 15 minutes
- Frozen storage requirement, listed for storage at -20°C and shipment on dry ice
Benefits
- Helps remove residual single-stranded primers after PCR before downstream molecular biology steps.
- Supports selective ssDNA digestion without treating the product simply as a general nuclease.
- The 10 U/µL concentration is practical for controlled enzyme addition in PCR clean-up workflows.
- The 5000 units pack size supports repeated use in laboratories performing routine DNA modification work.
Why Choose the Exonuclease I, 10 U/µL, 5000 Units (E70073X)?
Exonuclease I, 10 U/µL, 5000 Units (E70073X) provides a verified Cytiva enzyme option for selective single-stranded DNA digestion and PCR primer clean-up. John Morris Group supplies DNA modifying enzymes to support molecular and immunodiagnostic workflows across Australian laboratories.
